Quantitative procedure for enumeration of bifidobacteria

Quantitative procedure for enumeration of bifidobacteria.

A membrane filter technique has been developed for the enumeration of bifidobacteria in natural aquatic environments. The technique is quantitative, selective, and differential. The medium (YN-6) contains: yeast extract, 2.0 g; agar, 1.5 g; polypeptone peptone, 1.0 g; vitamin-free Casamino Acids, 0.8 g; sodium chloride, 0.32 g; and L-cysteine hydrochloride, 0.003 g; in 100 ml of deionized water...

Enumeration of bifidobacteria in animal intestinal samples

TPY agar supplemented with mupirocin (100 mg/liter) and glacial acetic acid (1 ml/liter) was effective in the enumeration of bifidobacteria in animal (11 species) fecal or intestinal samples. On the other hand, less than 50% of isolates from pig feces were identified as Bifidobacterium sp. using this medium. Subsequently TPY agar modified by the addition of mupirocin (100 mg/liter), glacial ace...

Human Fecal Samples for Quantification of Bifidobacteria in New Real-Time Quantitative PCR Procedure

Enumeration, Isolation, and Identification of Bifidobacteria from Dairy Products

Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg l). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46 °C was tested. Subs...

Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria

The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides-mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species commonly used to make fermented milks. Pure culture suspensions of a total of 13 dairy bacteria st...